Bacteriophages from waters of the Volga region

Abstract

One of the promising approaches to the treatment of infections caused by multidrug-resistant bacteria looks a therapy with bacteriophage. While bacteriophages are indifferent to bacterial resistance to antibiotics, bacteriophages are generally strain-specific, i.e., various isolates of the same bacterium can be insensitive to the given phage, that requires the creation of the library of bacteriophages. Here we report the isolation from wastewater from the city of Kazan (Republic of Tatarstan) the number of bacteriophages virulent against a number of opportunistic bacteria (Klebsiella pneumoniae, Escherichia coli, Cronobacter sakazakii and Enterococcus faecalis, Salmonella enterica Enteritidis and Typhimurium). Phages formed a clear plaques on the bacterial lawn and had a titer up to 10⁹ PFU/ml. Additionally, the polyvalent properties of bacteriophages were revealed: KES1, KES2 are lytic against C. sakazakii and S. enterica serovar Enteritidis, KST1 lyses S. enterica serovars Enteritidis and Typhimurium, KEF1 is virulent against E. faecalis and S. enterica serovar Enteritidis.