Antibiotics and phage resistant isolates of Enterobacteriaceae as a source of new infectious risks in surface water bodies

  • Zatsarinnaya E.A. 1
  • Lunkova E.S. 2
  • Trunyakova A.S. 3
  • Gaskova A.S. 1
  • Kuckir V.D. 4
  • 1 Scientific laboratory of evolutionary ecology, Ryazan state university named by S. A. Esenin, Svobody Str., 46, Ryazan, 390000, Russia
    2 Department of management and economics of pharmacy, Ryazan State Medical University named after academicial I.P. Pavlov, Vysokovoltnaya Str., 9, Ryazan, 390026, Russia
    3 Laboratory for plague microbiology, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow region, 142279, Russia
    4 Institute of Environmental and Agricultural Biology (X-Bio), University of Tyumen, Volodarskogo Str.,6, Tyumen, 625003, Russia
Keywords: antibiotic resistance, resistance to bacteriophages, family Enterobacteriaceae, surface freshwater bodies, public health risks.

Abstract

Freshwater environments are susceptible to possible bacterial contamination by various variants, including drug-resistant ones. Such pollution occurs either by direct intake of polluted wastewater or by surface runoff from the surrounding area. The existing quantitative sanitary and hygienic assessment of aquatic ecosystems is becoming less informative, as it underestimates the risks due to antibiotic-resistant bacterial strains. In order to assess the level of resistance to antibiotics and bacteriophages among enterobacteria, water samples were taken from five surface water bodies in Ryazan during the spring and summer periods of 2016 and 2017. It is established that the absolute majority of enterobacteria were multidrug-resistant (MDR) and more than 80% were resistant to the examined bacteriophage preparations. In addition, an increase in the occurrence of extremely resistant variants (XDR) among Enterobacteriaceae was active. About half of all isolated isolates produced Extended-Spectrum -Lactamases (ESBLs). The increase in resistance to bacteriophages occurs with an increase in the resistance of isolated enterobacteria to antibiotics.
Published
2020-09-09
Section
Articles