Experimental equipment for Lake Baikal deep biosphere microorganism’s exploration and some results obtained using this equipment

  • Manakov A.Yu. 1
  • Pavlova O.N. 2
  • Bukin S.V. 2
  • Kostyreva E.A. 3
  • Moskvin V.I. 3
  • Morozov I.V. 4, 5
  • Rodionova T.V. 1
  • Zemskaya T.I. 2
  • 1 Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Acad. Lavrentiev Ave., 3, 630 090 Novosibirsk, Russia
    2 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia
    3 Trofimuk Institute of Petroleum Geology and Geophysics, Siberian Branch of the Russian Academy of Sciences, Acad. Koptug Ave. 3, 630 090 Novosibirsk, Russia
    4 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Acad. Lavrentiev Ave., 8, 630 090 Novosibirsk, Russia
    5 Novosibirsk State University, Pirogov Str., 2, 630 090 Novosibirsk, Russia
Keywords: special autoclaves, thermobaric experiments, microbial communities, transformation of organic matter, bottom sediments, Lake Baikal

Abstract

The development of special autoclaves with automatic temperature control (Nikolaev Institute of Inorganic Chemistry) had enabled reproduction of Lake Baikal tectonically active zone typical conditions (80°C, 5 MPa). This article describes in details the equipment developed and demonstrates some results obtained using the equipment. In series of thermobaric experiments on the cultivation of bottom sediment microbial communities, we have determined the microbial community potential to transform organic matter via the formation of dibenzothiophenes, tri- and monoaromatic steroids as well as petroleum biomarkers (retene and gammacerene). The particular bottom sediment geochemical environment associated with hydrocarbon discharge imply the composition of microbial communities and, hence, the organic matter transformation degree as well as a compound spectrum resulting from the destruction of organic matter under thermobaric conditions. The presence of microorganisms with an unusual metabolism suggests the promising potential for such studies both in application to Lake Baikal and worldwide.

Published
2019-09-19
Section
Articles