Genomic characterization of Pseudomonas bacteriophage AN14

  • Evseev P.V. 1
  • Gorshkova A.S. 2
  • Sykilinda N.N. 1
  • Drucker V.V. 2
  • Miroshnikov K.A. 1
  • 1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia
    2 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia
Keywords: Bacteriophage, Pseudomonas, genomics, phylogeny, taxonomy


Siphoviruses with a prolate capsid infecting Pseudomonas sp. were isolated from environmental sources through the world, and were recently defined as a separate taxonomic genus Yuavirus. Besides a distinguishable morphology, the hallmarks of the genus are heavily modified genomic DNA and a lytic infection cycle while putative lysogeny genes are proposed in the genomes. Bacteriophage AN14 was isolated from Lake Baikal ecosystem and was assigned as a member of Yuavirus. We report the biological and morphological features if this phage, as well as the comprehensive re-annotation of its genome. Modern bioinformatics analysis resulted in the refinement of the taxonomic attributation of the YuA-like phages and highlighting the specific genomic and proteomic features typical for Yuavirus phages including AN14.