Characterization of photoautotrophic microbial communities in the coastal water of Lake Baikal

  • Belykh O.I. 1
  • Tikhonova I.V. 1
  • Krasnopeev A.Yu. 1
  • Galachyants A.D. 1
  • Sukhanova E.V. 1
  • Suslova M.Yu. 1
  • Gorshkova A.S. 1
  • Gevorgyan G. 2
  • Potapov S.A. 1
  • 1 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia
    2 Scientific Center of Zoology and Hydroecology, National Academy of Sciences of Republic of Armenia, Paruyr Sevak str. 7, 0014, Yerevan, Armenia
Keywords: cyanobacteria, algae, 16S rRNA, RuBisCO, high throughput sequencing, Lake Baikal


In this study, we determined the concentration of chlorophyll a in biofilms formed on rocky substrates, in the surface water microlayer, water column, and near-bottom water layer of the coastal zone of Lake Baikal. High throughput sequencing of 16S rRNA and rbcL gene libraries revealed high genetic and taxonomic diversity of photoautotrophic microbial communities in the studied ecotopes of the lake. The UPGMA analysis indicated that the composition of photoautotrophic communities largely depends on both the geographical location and ecotope. Using electron microscopy, we described the structure and composition of biofilms.