Reaction of inducible hsp70 form on different environmental conditions in Ommatogammarus flavus

  • Shirokova Yu.A. 1
  • Madyarova E.V. 1, 2
  • Shatilina Zh.M. 1, 2
  • Shmatov F.M. 1
  • Timofeyev M.A. 1, 2
  • 1 Irkutsk State University, Lenin Str., 3, Irkutsk, 664025, Russia
    2 Baikal Research Centre, Lenin Str., 21, Irkutsk, 664003, Russia
Keywords: Baikal, amphipods, adaptations, heat shock proteins, hydrostatic pressure, temperature

Abstract

The aim of the research was to estimate heat shock protein expression under the change in ambient temperature and hydrostatic pressure in Baikal deep-water amphipods Ommatogammarus flavus. The expression of the constitutive hsp70 transcript did not change in response to the influence of both environmental factors. The inducible hsp70 form did not differ after the change in hydrostatic pressure in amphipods captured from the depth of 50 and 750 m. High expression of inducible hsp70 transcript was shown during gradual increase in ambient temperature when it reached 22C. The detected increase in hsp70 expression is associated with the cells reaction to protein damage at the high temperature. Thus, the studied hsp70 inducible form is involved in the adaptation to change in ambient temperature in O. flavus despite the fact that this species inhabits the deep-water zone with stable temperature conditions. It is necessary to analyze other isoforms of hsp in the further studies to find those that participate in the adaptation to high hydrostatic pressure.

Published
2020-09-07
Section
Articles