Detection of mutagenic activity of Lake Sevan water using Trad-SHM and Trad-MCN bioassays
AbstractThe process of bioindication of freshwater ecosystems mutagenic effects using sensitive test-objects of plants is very appropriate and effective. We have investigated the genotoxicity and clastogenicity of deep water samples (24 m) in seven locations of Lake Sevan basin (Armenia) with the application of Trad-SHM (staimen hair mutation) and Trad-MCN (micronuclei in tetrads of microspores) bioassays using Tradescantia (clone 02). The study has revealed a significant increase in the frequency of somatic resessive mutation (RME) and increased amount of micronuclei in tetrads and tetrads with micronuclei in microspores of the plants submerged in the all studied water samples compared to the background. The significant positive correlation between the RME frequency and the concentration of some chemical elements Ni, Mo, Fe, Ca, Mg, K, Na in the studied water samples was shown. Also the positive correlation between tetrads with micronuclei frequency and the concentration of Cd, Ni, Mo, Fe, Ca, Mg, Na in the investigated water samples was shown.
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