Vital ﬂuorescent dyes for study of silicifying organisms
The mini-review describes application of vital ﬂuorescent dyes to study silica biosynthesis in diatom algae and siliceous sponges. Mechanisms of these processes are not clear and study of growing siliceous constructions in living organisms is complicated with silica transparency and presence of mature elements (siliceous valves of diatoms and spicules of sponges). Specifc ﬂuorescence dyes stain growing siliceous elements of skeletons and also primary siliceous particles which are formed during silicon capture from the environment. Vital ﬂuorescent dyes based on 7-nitro-2,1,3-benzoxadiazole, pyridyl oxazole and coumarin ﬂuorophores are described as well as examples of application of these dyes to study diatom algae and siliceous sponges.
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