Phylogenetic diversity of prokaryotes from the Churek-Dorgun cold spring (Tuva Republic)

Abstract

For the first time, the composition of microbial communities in the sediments of the cold freshwater spring Churek-Dorgun, located in the Kaa-Khem district of the Republic of Tuva, was studied using high-throughput sequencing. According to the chemical composition, the water of the Churek-Dorgun spring belonged to the sulfate-hydrocarbonate calcium type with weak mineralization (0.39 g / L) and neutral pH values (6.8 - 7.3). According to the sequencing results, 38,066 reads of 16S rRNA gene belonging to the Bacteria and Archaea domains were identified in the sediments sample. Archaea accounted for 0.27% of the microorganisms, with the phylum Methanobacteriota predominating. In total, 23 phyla in the bacterial microbial community were identified. Three bacterial phyla - Pseudomonadota, Bacillota, and Bacteroidota - which are typical of freshwater microbial communities dominated. The basis of the silt microbial community was widespread widespread in different ecosystems microorganisms: Aeromonas, Psychrosinus, Malikia, Dechloromonas, Sunxiuqinia, Thiobacillus, Geobacter, Litorilinea, Giesbergeria and Rivibacter. They have different physiological properties and metabolism. Microorganisms involved in the sulfur cycle were also found in the silt, including chemolithotrophic sulfur-oxidizing bacteria of the genus Thiobacillus (class Betaproteobacteria, 3.02%), microaerophilic sulfur-reducing bacteria of the genus Geobacter (class Deltaproteobacteria, 2.86%), and sulfate-reducing Deltaproteobacteria.