Genomic characterization of Pseudomonas bacteriophage AN14

Abstract

Siphoviruses with a prolate capsid infecting Pseudomonas sp. were isolated from environmental sources through the world, and were recently defined as a separate taxonomic genus Yuavirus. Besides a distinguishable morphology, the hallmarks of the genus are heavily modified genomic DNA and a lytic infection cycle while putative lysogeny genes are proposed in the genomes. Bacteriophage AN14 was isolated from Lake Baikal ecosystem and was assigned as a member of Yuavirus. We report the biological and morphological features if this phage, as well as the comprehensive re-annotation of its genome. Modern bioinformatics analysis resulted in the refinement of the taxonomic attributation of the YuA-like phages and highlighting the specific genomic and proteomic features typical for Yuavirus phages including AN14.